ANTIGEN-ANTIBODY REACTIONS IN ALLERGIC HUMAN TISSUES

Antigen-antibody Reactions in Allergic Human Tissues

Skin sensitizing human antibodies were conjugated with various fluorescent dyes without significant loss in their ability to combine with specific antigen in vitro. A biopsy of the skin site challenged with egg albumin in a patient sensitive to this antigen could be stained specifically by the fluorescent reagins. The epithelial cells of the epidermis, sweat glands, hair follicles, and sebaceou...

Antigen-antibody reactions Immunoagglutination Immunoprecipitation

Antigen – Antibody Reactions with Labeled Reagents

Assessment of heterogeneity in antibody-antigen displacement reactions.

The intrinsic binding characteristics of monoclonal antibodies are modified upon immobilization onto a solid-phase matrix. Factors such as the distribution in affinity must therefore be taken into consideration in order to predict the kinetics of antibody binding at solid-liquid interfaces. A mathematical analysis is presented herein that allows the assessment of heterogeneity in the affinity o...

Immunohistologic Studies on Antigen-antibody Reactions in the Avascular Cornea

The injection of antigen into the center of the avascular cornea of homologously sensitized animals induced a ring of opacification between the center of the cornea and the limbus. This ring of opacification was composed of a line of deeply eosinophilic amorphous material in a matrix of swollen collagen fibers, palisaded by polymorphonuclear leucocytes. By use of fluor-tagged antigen, it was sh...